• Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200
  • Western blot analysis of A549 3T3 mouse-liver SH-SY5Y K562 Hela 293T cells using TLE1/2/3/4 Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-TLE1/2/3/4 antibody (721-770 aa) (STJ98577)

SKU:
STJ98577

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transducin-like enhancer protein 1 and Transducin-like enhancer protein 2 and Transducin-like enhancer protein 3 and Transducin-like enhancer protein 4 (721-770 aa) is suitable for use in Western Blot, ELISA and Immuno
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLE4
TLE1
7091
7088
7090
TLE4_HUMAN
TLE1_HUMAN
TLE3_HUMAN
Immunogen Region: 721-770 aa
Specificity: TLE1/2/3/4 Polyclonal Antibody detects endogenous levels of TLE1/2/3/4
Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human TLE1/TLE2/TLE3/TLE4 at the amino acid range 721-770
Post Translational Modifications Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1. Ubiquitinated by XIAP/BIRC4.
Function Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
Protein Name Transducin-Like Enhancer Protein 1
E(Sp1 Homolog
Enhancer Of Split Groucho-Like Protein 1
Esg1
Database Links Reactome: R-HSA-201722
Reactome: R-HSA-2122947
Reactome: R-HSA-3769402
Reactome: R-HSA-4641265
Cellular Localisation Nucleus
Nuclear And Chromatin-Associated
Depending On Isoforms And Phosphorylation Status
Hyperphosphorylation Decreases The Affinity For Nuclear Components
Alternative Antibody Names Anti-Transducin-Like Enhancer Protein 1 antibody
Anti-E(Sp1 Homolog antibody
Anti-Enhancer Of Split Groucho-Like Protein 1 antibody
Anti-Esg1 antibody
Anti-TLE1 antibody

Information sourced from Uniprot.org

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