• Immunohistochemistry analysis of paraffin-embedded Human esophageal using TKT antibody (STJ28236) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat liver using TKT antibody (STJ28236) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using TKT Rabbit polyclonal antibody (STJ28236) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TKT antibody (1-280) (STJ28236)

SKU:
STJ28236

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TKT
Gene ID: 7086
Uniprot ID: TKT_HUMAN
Immunogen Region: 1-280
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TKT (NP_001128527.1).
Immunogen Sequence: MESYHKPDQQKLQALKDTAN RLRISSIQATTAAGSGHPTS CCSAAEIMAVLFFHTMRYKS QDPRNPHNDRFVLSKGHAAP ILYAVWAEAGFLAEAELLNL RKISSDLDGHPVPKQAFTDV ATGSLGQGLGAACGMAYTGK YFDKASYRVYCLLGDGELSE GSVWEAMAFASIYKLDNLVA ILDINRLGQSDPAPLQHQMD IYQKRCEAFGWHAIIVDGHS VEELCKAFGQAKHQPTAII
Function Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.
Protein Name Transketolase
Tk
Database Links Reactome: R-HSA-163754
Reactome: R-HSA-71336
Reactome: R-HSA-9818028
Alternative Antibody Names Anti-Transketolase antibody
Anti-Tk antibody
Anti-TKT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance