Anti-TIRAP antibody (1-221) (STJ25855)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TIRAP (1-221) antibody for use in IHC-P and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TIRAP |
| Gene ID | 114609 |
| Uniprot ID | TIRAP_HUMAN |
| Immunogen Region | 1-221 |
| Immunogen Sequence | MASSTSLPAPGSRPKKPLGK MADWFRQTLLKKPKKRPNSP ESTSSDASQPTSQDSPLPPS LSSVTSPSLPPTHASDSGSS RWSKDYDVCVCHSEEDLVAA QDLVSYLEGSTASLRCFLQL RDATPGGAIVSELCQALSSS HCRVLLITPGFLQDPWCKYQ MLQALTEAPGAEGCTIPLLS GLSRAAYPPELRFMYYVDGR GPDGGFRQVKEAVMRYLQTL S |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human TIRAP (NP_001034750.1). |
Additional Info
| Tissue Specificity | Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. |
| Post Translational Modifications | Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation. |
| Function | Adapter involved in TLR2, TLR4 and RAGE signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha (TNF) and interleukin-6 (IL6). |
| Protein Name | Toll/Interleukin-1 Receptor Domain-Containing Adapter ProteinTir Domain-Containing Adapter ProteinAdaptor Protein WyattMyd88 Adapter-Like ProteinMyd88-2 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-166058Reactome: R-HSA-5602498Reactome: R-HSA-5603041 |
| Cellular Localisation | CytoplasmCell MembraneMembraneColocalizes With Dab2ip At The Plasma Membrane |
| Alternative Antibody Names | Anti-Toll/Interleukin-1 Receptor Domain-Containing Adapter Protein antibodyAnti-Tir Domain-Containing Adapter Protein antibodyAnti-Adaptor Protein Wyatt antibodyAnti-Myd88 Adapter-Like Protein antibodyAnti-Myd88-2 antibodyAnti-TIRAP antibodyAnti-MAL antibody |
Information sourced from Uniprot.org