• Immunohistochemistry analysis of paraffin-embedded Mouse pancreatic islet using TIMP2 antibody (STJ118867) at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human breast cancer using TIMP2 antibody (STJ118867) at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat ovary using TIMP2 antibody (STJ118867) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using TIMP2 antibody (STJ118867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TIMP2 antibody (100-200) (STJ118867)

SKU:
STJ118867

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TIMP2 (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIMP2
Gene ID: 7077
Uniprot ID: TIMP2_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TIMP2 (NP_003246.1).
Immunogen Sequence: VSLDVGGKKEYLIAGKAEGD GKMHITLCDFIVPWDTLSTT QKKSLNHRYQMGCECKITRC PMIPCYISSPDECLWMDWVT EKNINGHQAKFFACIKRSDG S
Post Translational Modifications The activity of TIMP2 is dependent on the presence of disulfide bonds.
Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Protein Name Metalloproteinase Inhibitor 2
Csc-21k
Tissue Inhibitor Of Metalloproteinases 2
Timp-2
Database Links Reactome: R-HSA-1592389
Reactome: R-HSA-6798695
Cellular Localisation Secreted
Alternative Antibody Names Anti-Metalloproteinase Inhibitor 2 antibody
Anti-Csc-21k antibody
Anti-Tissue Inhibitor Of Metalloproteinases 2 antibody
Anti-Timp-2 antibody
Anti-TIMP2 antibody

Information sourced from Uniprot.org

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