Anti-TIGIT antibody [DM178] (STJA0038192)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant human TIGIT protein
General Information
| Short Description | Rabbit monoclonal anti-TIGIT for use in FC and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | DM178 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | FC 1:50-1:100ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | TIGIT |
| Gene ID | 201633 |
| Uniprot ID | TIGIT_HUMAN |
| Immunogen | Recombinant human TIGIT protein |
Additional Info
| Tissue Specificity | Expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). |
| Function | Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway. Also acts as a receptor for NECTIN4 to inhibit NK cell cytotoxicity. |
| Protein Name | T-Cell Immunoreceptor With Ig And Itim DomainsV-Set And Immunoglobulin Domain-Containing Protein 9V-Set And Transmembrane Domain-Containing Protein 3 |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinClustered To The Immunological Synapse Where It Disrupts Granule Polarization And Cytotoxicity Of Nk Cells Once Engaged With Pvr |
| Alternative Antibody Names | Anti-T-Cell Immunoreceptor With Ig And Itim Domains antibodyAnti-V-Set And Immunoglobulin Domain-Containing Protein 9 antibodyAnti-V-Set And Transmembrane Domain-Containing Protein 3 antibodyAnti-TIGIT antibodyAnti-VSIG9 antibodyAnti-VSTM3 antibody |
Information sourced from Uniprot.org