Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4ยฐC overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-TIE1 antibody (851-900 aa) (STJ96014)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TIE1 at the amino acid range 851-900
STJ96014
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General Information

Short DescriptionRabbit polyclonal anti-Tyrosine-protein kinase receptor Tie-1 (851-900 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeIHC-P 100-300
WB 1:500-1:2000
ELISA 1:10000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTIE1
Gene ID7075
Uniprot IDTIE1_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TIE1 at the amino acid range 851-900
Immunogen Region851-900 aa
SpecificityTie-1 Polyclonal Antibody detects endogenous levels of Tie-1 protein.

Additional Info

Post Translational Modifications Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.
Function Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
Protein Name Tyrosine-Protein Kinase Receptor Tie-1
Database Links
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Receptor Tie-1 antibody
Anti-TIE1 antibody
Anti-TIE antibody

Information sourced from Uniprot.org

Citations

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