Anti-TIAM 1 antibody (STJ13100389)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from human TIAM1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse.
General Information
| Short Description | Rabbit polyclonal anti-TIAM 1 for use in IHC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | TIAM1 |
| Gene ID | 7074 |
| Uniprot ID | TIAM1_HUMAN |
| Immunogen | A synthetic peptide from human TIAM1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse. |
| Specificity | Specific for TIAM1. |
Additional Info
| Post Translational Modifications | Ubiquitinated. Undergoes 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 by a CUL3(KBTBD6/7) E3 ubiquitin ligase complex composed of CUL3, RBX1, KBTBD6 and KBTBD7. 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 triggers proteasomal degradation. Ubiquitination at Lys-1404 and Lys-1420 by CUL3(KBTBD6/7) also requires the membrane-associated protein GABARAP and may therefore be spatially restricted within the cell. |
| Function | Guanyl-nucleotide exchange factor that activates RHO-like proteins and connects extracellular signals to cytoskeletal activities. Activates RAC1, CDC42, and to a lesser extent RHOA and their downstream signaling to regulate processes like cell adhesion and cell migration. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor Tiam1T-Lymphoma Invasion And Metastasis-Inducing Protein 1Tiam-1 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-3928662Reactome: R-HSA-3928665Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423Reactome: R-HSA-9032845 |
| Cellular Localisation | Cell JunctionCell MembranePeripheral Membrane ProteinCytoplasmic SideDetected At The Boundary Between Cells With Actin-Rich ProtrusionsPresence Of Krit1Cdh5 And Rap1b Is Required For Its Localization To The Cell Junction |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor Tiam1 antibodyAnti-T-Lymphoma Invasion And Metastasis-Inducing Protein 1 antibodyAnti-Tiam-1 antibodyAnti-TIAM1 antibody |
Information sourced from Uniprot.org