• Immunohistochemistry analysis of paraffin-embedded mouse lung using THRA Rabbit polyclonal antibody (STJ27561) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of SH-SY5Y cells, using THRA antibody (STJ27561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-THRA antibody (138-489) (STJ27561)

SKU:
STJ27561

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-THRA (138-489) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: THRA
Gene ID: 7067
Uniprot ID: THA_HUMAN
Immunogen Region: 138-489
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 138-489 of human THRA (NP_003241.2).
Immunogen Sequence: IEQNRERRRKEEMIRSLQQR PEPTPEEWDLIHIATEAHRS TNA
Function Isoform Alpha-1: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. Isoform Alpha-2: Does not bind thyroid hormone and functions as a weak dominant negative inhibitor of thyroid hormone action.
Protein Name Thyroid Hormone Receptor Alpha
Nuclear Receptor Subfamily 1 Group A Member 1
V-Erba-Related Protein 7
Ear-7
C-Erba-1
C-Erba-Alpha
Database Links Reactome: R-HSA-383280
Reactome: R-HSA-4090294 P10827-2
Cellular Localisation Nucleus
Isoform Alpha-2: Cytoplasm
When Overexpressed Found In The Cytoplasm Where It Colocalizes With Tacc1
Alternative Antibody Names Anti-Thyroid Hormone Receptor Alpha antibody
Anti-Nuclear Receptor Subfamily 1 Group A Member 1 antibody
Anti-V-Erba-Related Protein 7 antibody
Anti-Ear-7 antibody
Anti-C-Erba-1 antibody
Anti-C-Erba-Alpha antibody
Anti-THRA antibody
Anti-EAR7 antibody
Anti-ERBA1 antibody
Anti-NR1A1 antibody
Anti-THRA1 antibody
Anti-THRA2 antibody

Information sourced from Uniprot.org

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