Host: | Mouse |
Applications: | IHC-P |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Mouse monoclonal antibody anti-TG is suitable for use in Immunohistochemistry research applications. |
Clonality : | Monoclonal |
Clone ID : | 2H11+6E1 |
Conjugation: | Unconjugated |
Isotype: | IgG1/Kappa + IgG1/Kappa |
Formulation: | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
Purification: | Affinity purified |
Dilution Range: | 1:100‐200 |
Storage Instruction: | Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
Gene Symbol: | TG |
Gene ID: | 7038 |
Uniprot ID: | THYG_HUMAN |
Specificity: | Positive control: Thyroid |
Immunogen: | Purified human thyroglobulin |
Tissue Specificity | Specifically expressed in the thyroid gland. |
Post Translational Modifications | Iodinated on tyrosine residues by TPO. There are 4 pairs of iodinated tyrosines used for coupling: acceptor Tyr-24 is coupled to donor Tyr-149 or Tyr-234, acceptor Tyr-2573 is coupled to donor Tyr-2540, acceptor Tyr-2766 in monomer 1 is coupled to donor Tyr-2766 in monomer 2 and acceptor Tyr-1310 in monomer 1 is coupled to donor Tyr-108 in monomer 2. Sulfated tyrosines are desulfated during iodination. Undergoes sequential proteolysis by cathepsins to release thyroxine (T4) and triiodothyronine (T3) hormones. In the thyroid follicle lumen, cross-linked TG (storage form) is solubilized by limited proteolysis mediated by cathepsins CTSB and/or CTSL. Partially cleaved TG is further processed by CTSK/cathepsin K and/or CTSL resulting in the release of T4. Following endocytosis, further processing occurs leading to the release of T3 and more T4 hormones. |
Function | Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling in the thyroid follicle lumen. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules. |
Protein Name | Thyroglobulin Tg |
Cellular Localisation | Secreted Secreted Into The Thyroid Follicle Lumen Localizes To Colloid Globules A Structure Formed In The Thyroid Follicle Lumen Consisting Of Cross-Linked Tg Arranged In Concentric Layers |
Alternative Antibody Names | Anti-Thyroglobulin antibody Anti-Tg antibody Anti-TG antibody |
Information sourced from Uniprot.org