| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TERT (550-600 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Tert |
| Gene ID: |
21752 |
| Uniprot ID: |
TERT_MOUSE |
| Immunogen Region: |
550-600 aa |
| Specificity: |
Specific for TERT. |
| Immunogen: |
A synthetic peptide from aa region 550-600 of mouse Telomerase reverse transcriptase (TERT) conjugated to blue carrier protein was used as the antigen. |
| Post Translational Modifications | Phosphorylation at Tyr-697 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation by the AKT pathway promotes nuclear location. Phosphorylation at the G2/M phase at Ser-447 by DYRK2 promotes ubiquitination by the EDVP complex and degradation. Ubiquitinated by the EDVP complex, a E3 ligase complex following phosphorylation at Ser-447 by DYRK2. Ubiquitinated leads to proteasomal degradation. |
| Function | Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis. |
| Protein Name | Telomerase Reverse TranscriptaseTelomerase Catalytic Subunit |
| Database Links | Reactome: R-MMU-171319Reactome: -MMU-201722 |
| Cellular Localisation | NucleusNucleolusNucleoplasmChromosomeTelomereCytoplasmPml BodyShuttling Between Nuclear And Cytoplasm Depends On Cell CyclePhosphorylation StatesTransformation And Dna DamageDiffuse Localization In The NucleoplasmEnriched In Nucleoli Of Certain Cell TypesTranslocated To The Cytoplasm Via Nuclear Pores In A Crm1/Ran-Dependent Manner Involving Oxidative Stress-Mediated Phosphorylation At Tyr-697Dephosphorylation At This Site By Shp2 Retains Tert In The NucleusTranslocated To The Nucleus By Phosphorylation By Akt |
| Alternative Antibody Names | Anti-Telomerase Reverse Transcriptase antibodyAnti-Telomerase Catalytic Subunit antibodyAnti-Tert antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
