Anti-TDP1 antibody (Internal) (STJ73309)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-TDP1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 75kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 68.4kDa according to NP_060789.2). Recommended concentration: 1-3µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | TDP1 |
| Gene ID | 55775 |
| Uniprot ID | TYDP1_HUMAN |
| Accession Number | NP_060789.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | PNDGTAQRTENHG |
| Specificity | Reported variants represent identical protein: NP_001008744.1, NP_060789.2 |
Additional Info
| Post Translational Modifications | Phosphorylated on serine and/or threonine residues, but not on tyrosine residues. |
| Function | DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. |
| Protein Name | Tyrosyl-Dna Phosphodiesterase 1Tyr-Dna Phosphodiesterase 1 |
| Database Links | Reactome: R-HSA-5693571 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Tyrosyl-Dna Phosphodiesterase 1 antibodyAnti-Tyr-Dna Phosphodiesterase 1 antibodyAnti-TDP1 antibody |
Information sourced from Uniprot.org