Anti-TDG antibody (1-410aa) [AT2F7] (STJA0041650)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenRecombinant human TDG (1-410aa) purified from E. coli
General Information
| Short Description | Mouse monoclonal anti-TDG (1-410aa) for use in ELISA, WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC/IF |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | AT2F7 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | By protein-A affinity chromatography |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TDG |
| Gene ID | 6996 |
| Uniprot ID | TDG_HUMAN |
| Accession Number | AAH37557 |
| Immunogen | Recombinant human TDG (1-410aa) purified from E. coli |
| Immunogen Region | 1-410aa |
Additional Info
| Post Translational Modifications | Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA. |
| Function | DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. |
| Protein Name | G/T Mismatch-Specific Thymine Dna GlycosylaseThymine-Dna GlycosylaseHtdg |
| Database Links | Reactome: R-HSA-110328Reactome: R-HSA-110329Reactome: R-HSA-110357Reactome: R-HSA-3108214Reactome: R-HSA-5221030 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-G/T Mismatch-Specific Thymine Dna Glycosylase antibodyAnti-Thymine-Dna Glycosylase antibodyAnti-Htdg antibodyAnti-TDG antibody |
Information sourced from Uniprot.org