Anti-TCOF1 antibody (659-958) (STJ25798)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TCOF1 (659-958) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TCOF1 |
| Gene ID | 6949 |
| Uniprot ID | TCOF_HUMAN |
| Immunogen Region | 659-958 |
| Immunogen Sequence | VRVGTQAPRKAGTATSPAGS SPAVAGGTQRPAEDSSSSEE SDSEEEKTGLAVTVGQAKSV GKGLQVKAASVPVKGSLGQG TAPVLPGKTGPTVTQVKAEK QEDSESSEEESDSEEAAASP AQVKTSVKKTQAKANPAAAR APSAKGTISAPGKVVTAAAQ AKQRSPSKVKPPVRNPQNST VLARGPASVPSVGKAVATAA QAQTGPEEDSGSSEEESDSE EEAETLAQVKPSGKTHQIR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 659-958 of human TCOF1 (NP_001008657.1). |
Additional Info
| Post Translational Modifications | Ubiquitinated. Monoubiquitination by the BCR(KBTBD8) complex promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Pyrophosphorylated by 5-diphosphoinositol pentakisphosphate (5-IP7). Serine pyrophosphorylation is achieved by Mg(2+)-dependent, but enzyme independent transfer of a beta-phosphate from a inositol pyrophosphate to a pre-phosphorylated serine residue. |
| Function | Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. |
| Protein Name | Treacle ProteinTreacher Collins Syndrome Protein |
| Database Links | |
| Cellular Localisation | NucleusNucleolus |
| Alternative Antibody Names | Anti-Treacle Protein antibodyAnti-Treacher Collins Syndrome Protein antibodyAnti-TCOF1 antibody |
Information sourced from Uniprot.org