Anti-TCF7L2 antibody (STJA0006832)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human TCF4/TCF7L2
General Information
| Short Description | Rabbit polyclonal anti-Transcription factor 7-like 2 for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TCF7L2 |
| Gene ID | 6934 |
| Uniprot ID | TF7L2_HUMAN |
| Immunogen | Synthesized peptide derived from human TCF4/TCF7L2 |
| Specificity | This antibody detects endogenous levels of TCF4/TCF7L2 at Human, Mouse |
Additional Info
| Post Translational Modifications | In vitro, phosphorylated by TNIK. Phosphorylated at Thr-201 and/or Thr-212 by NLK. Phosphorylation by NLK at these sites inhibits DNA-binding by TCF7L2/TCF4, thereby preventing transcriptional activation of target genes of the canonical Wnt/beta-catenin signaling pathway. Polysumoylated. Sumoylation is enhanced by PIAS family members and desumoylation is enhanced by SENP2. Sumoylation/desumoylation regulates TCF7L2/TCF4 transcription activity in the Wnt/beta-catenin signaling pathway without altering interaction with CTNNB1 nor binding to DNA. |
| Function | Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine. |
| Protein Name | Transcription Factor 7-Like 2Hmg Box Transcription Factor 4T-Cell-Specific Transcription Factor 4T-Cell Factor 4Tcf-4Htcf-4 |
| Database Links | Reactome: R-HSA-201722Reactome: R-HSA-3769402Reactome: R-HSA-381771Reactome: R-HSA-4086398Reactome: R-HSA-4411364Reactome: R-HSA-4641265Reactome: R-HSA-5339700Reactome: R-HSA-8853884Reactome: R-HSA-8951430Reactome: R-HSA-9823730Reactome: R-HSA-9825892 |
| Cellular Localisation | NucleusPml BodyDiffuse PatternColocalizes With Sumo1 And Pias4 In A Subset Of Pml (Promyelocytic Leukemia) Nuclear Bodies |
| Alternative Antibody Names | Anti-Transcription Factor 7-Like 2 antibodyAnti-Hmg Box Transcription Factor 4 antibodyAnti-T-Cell-Specific Transcription Factor 4 antibodyAnti-T-Cell Factor 4 antibodyAnti-Tcf-4 antibodyAnti-Htcf-4 antibodyAnti-TCF7L2 antibodyAnti-TCF4 antibody |
Information sourced from Uniprot.org