Western blot analysis of lysates from HCT 116 cells, using TCF4 Rabbit polyclonal antibody (STJ11102820) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TCF4 antibody (1-210) (STJ11102820)

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STJ11102820
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TCF4
Gene ID: 6925
Uniprot ID: ITF2_HUMAN
Immunogen Region: 1-210
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human TCF4 (NP_003190.1).
Immunogen Sequence: MHHQQRMAALGTDKELSDLL DFSAMFSPPVSSGKNGPTSL ASGHFTGSNVEDRSSSGSWG NGGHPSPSRNYGDGTPYDHM TSRDLGSHDNLSPPFVNSRI QSKTERGSYSSYGRESNLQG CHQQSLLGGDMDMGNPGTLS PTKPGSQYYQYSSNNPRRRP LHSSAMEVQTKKVRKVPPGL PSSVYAPSASTADYNRDSPG YPSSKPATST
Tissue Specificity Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.
Function Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
Protein Name Transcription Factor 4
Tcf-4
Class B Basic Helix-Loop-Helix Protein 19
Bhlhb19
Immunoglobulin Transcription Factor 2
Itf-2
Sl3-3 Enhancer Factor 2
Sef-2
Database Links Reactome: R-HSA-525793
Reactome: R-HSA-9839394
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor 4 antibody
Anti-Tcf-4 antibody
Anti-Class B Basic Helix-Loop-Helix Protein 19 antibody
Anti-Bhlhb19 antibody
Anti-Immunoglobulin Transcription Factor 2 antibody
Anti-Itf-2 antibody
Anti-Sl3-3 Enhancer Factor 2 antibody
Anti-Sef-2 antibody
Anti-TCF4 antibody
Anti-BHLHB19 antibody
Anti-ITF2 antibody
Anti-SEF2 antibody

Information sourced from Uniprot.org

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