Anti-TCF3/ITF1, Biotinylated antibody (Internal) {Biotin} (STJ73204)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationBiotin
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-TCF3/ITF1, Biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). See non-biotinylated parental product's datasheet for further QC data. Re |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | TCF3 |
| Gene ID | 6929 |
| Uniprot ID | TFE2_HUMAN |
| Accession Number | NP_003191.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | KAPRARTSPDEDED. |
| Specificity | This antibody is expected to recognize reported isoform E12 (NP_003191.1) only. |
Additional Info
| Function | Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Together with TCF15, required for the mesenchymal to epithelial transition. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region. Isoform E47: Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity. |
| Protein Name | Transcription Factor E2-AlphaClass B Basic Helix-Loop-Helix Protein 21Bhlhb21Immunoglobulin Enhancer-Binding Factor E12/E47Immunoglobulin Transcription Factor 1Kappa-E2-Binding FactorTranscription Factor 3Tcf-3Transcription Factor Itf-1 |
| Database Links | Reactome: R-HSA-525793Reactome: R-HSA-8939236Reactome: R-HSA-9839394 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E2-Alpha antibodyAnti-Class B Basic Helix-Loop-Helix Protein 21 antibodyAnti-Bhlhb21 antibodyAnti-Immunoglobulin Enhancer-Binding Factor E12/E47 antibodyAnti-Immunoglobulin Transcription Factor 1 antibodyAnti-Kappa-E2-Binding Factor antibodyAnti-Transcription Factor 3 antibodyAnti-Tcf-3 antibodyAnti-Transcription Factor Itf-1 antibodyAnti-TCF3 antibodyAnti-BHLHB21 antibodyAnti-E2A antibodyAnti-ITF1 antibody |
Information sourced from Uniprot.org