| Host: | Goat |
| Applications: | Pep-ELISA/IF/FC |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-TCF1/HNF1 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000.WB: A customer reported a weak band at approx. 75kDa on lysate of cell line Caco-2, at a concentration of 3ug/ml. Primary incubation 1 hour at room temperature.IF: Strong expression of |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | HNF1A |
| Gene ID: | 6927 |
| Uniprot ID: | HNF1A_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000536.3 |
| Immunogen Sequence: | DELPTKKGRRNRFK |
| Post Translational Modifications | Ubiquitinated in s SPOP-dependent manner.leading to prteasomal degradation. |
| Function | Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Activates the transcription of CYP1A2, CYP2E1 and CYP3A11. (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with NR5A2 to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII). |
| Protein Name | Hepatocyte Nuclear Factor 1-AlphaHnf-1-AlphaHnf-1aLiver-Specific Transcription Factor Lf-B1Lfb1Transcription Factor 1Tcf-1 |
| Database Links | Reactome: R-HSA-210745 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Hepatocyte Nuclear Factor 1-Alpha antibodyAnti-Hnf-1-Alpha antibodyAnti-Hnf-1a antibodyAnti-Liver-Specific Transcription Factor Lf-B1 antibodyAnti-Lfb1 antibodyAnti-Transcription Factor 1 antibodyAnti-Tcf-1 antibodyAnti-HNF1A antibodyAnti-TCF1 antibody |
Information sourced from Uniprot.org