• Western blot analysis of extracts of various cell lines, using TARS2 antibody (STJ114719) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TARS2 antibody (100-400) (STJ114719)

SKU:
STJ114719

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TARS2
Gene ID: 80222
Uniprot ID: SYTM_HUMAN
Immunogen Region: 100-400
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TARS2 (NP_079426.2).
Immunogen Sequence: VNGEPYDLERPLETDSDLRF LTFDSPEGKAVFWHSSTHVL GAAAEQFLGAVLCRGPSTEY GFYHDFFLGKERTIRGSELP VLERICQELTAAARPFRRLE ASRDQLRQLFKDNPFKLHLI EEKVTGPTATVYGCGTLVDL CQGPHLRHTGQIGGLKLLSN SSSLWRSSGAPETLQRVSGI SFPTTELLRVWEAWREEAEL RDHRRIGKEQELFFFHELSP GSCFFLPRGTRVYNALVAF
Function Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain.
Protein Name Threonine--Trna Ligase - Mitochondrial
Threonyl-Trna Synthetase
Thrrs
Threonyl-Trna Synthetase-Like 1
Database Links Reactome: R-HSA-379726
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Threonine--Trna Ligase - Mitochondrial antibody
Anti-Threonyl-Trna Synthetase antibody
Anti-Thrrs antibody
Anti-Threonyl-Trna Synthetase-Like 1 antibody
Anti-TARS2 antibody
Anti-TARSL1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance