Anti-TANK antibody (150-200 aa) (STJ13102539)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 150-200 of human TANK conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-TANK (150-200 aa) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | TANK |
| Gene ID | 10010 |
| Uniprot ID | TANK_HUMAN |
| Immunogen | A synthetic peptide from aa region 150-200 of human TANK conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | 150-200 aa |
| Specificity | Specific for TANK. |
Additional Info
| Post Translational Modifications | Phosphorylated by IKBKE. (Microbial infection) Cleaved by encephalomyocarditis virus (EMCV) protease 3C. This cleavage allows the virus to disrupt the TANK-TBK1-IKKepsilon-IRF3 complex, thereby inhibiting the induction of the IFN-beta signal pathway. (Microbial infection) Cleaved by Seneca Valley virus protease 3C allowing the virus to suppress interferon type-I through both RIG-I and Toll-like receptor-dependent pathways. |
| Function | Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage. Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage. May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. |
| Protein Name | Traf Family Member-Associated Nf-Kappa-B ActivatorTraf-Interacting ProteinI-Traf |
| Database Links | Reactome: R-HSA-9013973Reactome: R-HSA-933541Reactome: R-HSA-936964Reactome: R-HSA-9824878Reactome: R-HSA-9828211 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Traf Family Member-Associated Nf-Kappa-B Activator antibodyAnti-Traf-Interacting Protein antibodyAnti-I-Traf antibodyAnti-TANK antibodyAnti-ITRAF antibodyAnti-TRAF2 antibody |
Information sourced from Uniprot.org