Anti-TAB1 antibody (N-Term) [3D10-G10-B6] (STJ99124)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
ImmunogenPurified recombinant human TAB1 (N-terminus) protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 (N-Term) for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 3D10-G10-B6 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TAB1 |
| Gene ID | 10454 |
| Uniprot ID | TAB1_HUMAN |
| Immunogen | Purified recombinant human TAB1 (N-terminus) protein fragments expressed in E.coli. |
| Immunogen Region | N-Term |
| Specificity | This antibody detects endogenous levels of TAB1 (N-terminus) and does not cross-react with related proteins. |
Additional Info
| Post Translational Modifications | Phosphorylated at all three sites Ser-423, Thr-431 and Ser-438 by MAPK14 when cells were exposed to cellular stresses, or stimulated with TNF-alpha, IL1 or LPS. These phosphorylations inhibit TAK1 activation by a feedback control mechanism. Dephosphorylated by DUSP14 at Ser-438, leading to TAB1-MAP3K7/TAK1 complex inactivation in T-cells. Ubiquitinated by MAP3K1 with 'Lys-63'-linked polyubiquitin.leading to activation of TAK1 and of JNK and p38 MAP kinases following EGF and TGF-beta stimulation. Ubiquitinated by ITCH with 'Lys-48'-linked polyubiquitin.leading to proteasomal degradation. Ubiquitinated by RNF114 during maternal-to-zygotic transition.leading to degradation. (Microbial infection) Deubiquitinated by Y.enterocolitica YopP. O-GlcNAcylated at Ser-395 by OGT is required for full MAP3K7/TAK1 activation upon stimulation with IL-1 or osmotic stress. Deglycosylated at Ser-395 by OGA. |
| Function | Key adapter protein that plays an essential role in JNK and NF-kappa-B activation and proinflammatory cytokines production in response to stimulation with TLRs and cytokines. Mechanistically, associates with the catalytic domain of MAP3K7/TAK1 to trigger MAP3K7/TAK1 autophosphorylation leading to its full activation. Similarly, associates with MAPK14 and triggers its autophosphorylation and subsequent activation. In turn, MAPK14 phosphorylates TAB1 and inhibits MAP3K7/TAK1 activation in a feedback control mechanism. Also plays a role in recruiting MAPK14 to the TAK1 complex for the phosphorylation of the TAB2 and TAB3 regulatory subunits. |
| Protein Name | Tgf-Beta-Activated Kinase 1 And Map3k7-Binding Protein 1Mitogen-Activated Protein Kinase Kinase Kinase 7-Interacting Protein 1Tgf-Beta-Activated Kinase 1-Binding Protein 1Tak1-Binding Protein 1 |
| Database Links | Reactome: R-HSA-168638Reactome: R-HSA-2871837Reactome: R-HSA-445989Reactome: R-HSA-450302Reactome: R-HSA-450321Reactome: R-HSA-5357956Reactome: R-HSA-5607764Reactome: R-HSA-5689880Reactome: R-HSA-9014325Reactome: R-HSA-9020702Reactome: R-HSA-937042Reactome: R-HSA-937072Reactome: R-HSA-9645460Reactome: R-HSA-9705671Reactome: R-HSA-975163 |
| Cellular Localisation | CytoplasmCytosolEndoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideRecruited To The Endoplasmic Reticulum Following Interaction With Sting1 |
| Alternative Antibody Names | Anti-Tgf-Beta-Activated Kinase 1 And Map3k7-Binding Protein 1 antibodyAnti-Mitogen-Activated Protein Kinase Kinase Kinase 7-Interacting Protein 1 antibodyAnti-Tgf-Beta-Activated Kinase 1-Binding Protein 1 antibodyAnti-Tak1-Binding Protein 1 antibodyAnti-TAB1 antibodyAnti-MAP3K7IP1 antibody |
Information sourced from Uniprot.org