Anti-T-bet/Tbx21 antibody (322-535 aa) [SRM5468] (STJ11107386)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal T-bet/Tbx21 (322-535 aa) antibody for use in WB, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5468 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 ยตg/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TBX21 |
| Gene ID | 30009 |
| Uniprot ID | TBX21_HUMAN |
| Immunogen Region | 322-535 aa |
| Immunogen Sequence | KGFRENFESMYTSVDTSIPS PPGPNCQFLGGDHYSPLLPN QYPVPSRFYPDLPGQAKDVV PQAYWLGAPRDHSYEAEFRA VSMKPAFLPSAPGPTMSYYR GQEVLAPGAGWPVAPQYPPK MGPASWFRPMRTLPMEPGPG GSEGRGPEDQGPPLVWTEIA PIRPESSDSGLGEGDSKRRR VSPYPSSGDSSSPAGAPSPF DKEAEGQFYNYFPN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 322-535 of human T-bet/Tbx21 (NP_037483.1). |
Additional Info
| Tissue Specificity | T-cell specific. |
| Post Translational Modifications | Phosphorylations at Ser-53, Tyr-77, Ser-225 and Ser-513 are regulated by mTORC1. Phosphorylation at Tyr-530 is essential for its interaction GATA3. Phosphorylation at Tyr-220, Tyr-266 and Tyr-305 enhances its transcriptional activator activity. Phosphorylation at Thr-303 is required for its interaction with NFATC2. Ubiquitinated at Lys-314, leading to its degradation by the proteasome. Ubiquitination is essential for controlling protein stability, binding to the T-box-binding element of the IFN-gamma promoter, and for interaction with NFATC2 through induction of phosphorylation at Thr-303. Deubiquitinated by USP10 leading to its stabilization. |
| Function | Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs. Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Induces permissive chromatin accessibilty and CpG methylation in IFNG. Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation. Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells. Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL- 13, via repression of transcriptional regulators GATA3 and NFATC2. Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN-stimulated genes. Acts as a regulator of antiviral B-cell responses.controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program. Required for the correct development of natural killer (NK) and mucosal-associated invariant T (MAIT) cells. |
| Protein Name | T-Box Transcription Factor Tbx21T-Box Protein 21T-Cell-Specific T-Box Transcription Factor T-BetTranscription Factor Tblym |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-T-Box Transcription Factor Tbx21 antibodyAnti-T-Box Protein 21 antibodyAnti-T-Cell-Specific T-Box Transcription Factor T-Bet antibodyAnti-Transcription Factor Tblym antibodyAnti-TBX21 antibodyAnti-TBET antibodyAnti-TBLYM antibody |
Information sourced from Uniprot.org