• Immunofluorescence analysis of L929 cells using SYT12 Rabbit polyclonal antibody (STJ11102890) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse brain, using SYT12 Rabbit polyclonal antibody (STJ11102890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-SYT12 antibody (95-200) (STJ11102890)

SKU:
STJ11102890

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SYT12
Gene ID: 91683
Uniprot ID: SYT12_HUMAN
Immunogen Region: 95-200
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 95-200 of human SYT12 (NP_001171351.1).
Immunogen Sequence: KGSLSIEDTFESISELGPLE LMGRELDLAPYGTLRKSQSA DSLNSISSVSNTFGQDFTLG QVEVSMEYDTASHTLNVAVM QGKDLLEREEASFESCFMRV SLLPDE
Post Translational Modifications Phosphorylation of Ser-97 is required for mossy-fiber long-term potentiation.
Function Synaptic vesicle phosphoprotein that enhances spontaneous neurotransmitter release but does not effect induced neurotransmitter release. Unlike other synaptotagmins, it does not bind Ca(2+) or phospholipids. Essential for mossy-fiber long-term potentiation in the hippocampus.
Protein Name Synaptotagmin-12
Synaptotagmin Xii
Sytxii
Database Links Reactome: R-HSA-6794361
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Synaptotagmin-12 antibody
Anti-Synaptotagmin Xii antibody
Anti-Sytxii antibody
Anti-SYT12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance