• Western blot analysis of extracts of various cell lines, using Synaptophysin antibody (STJ28429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded human brain using Synaptophysin rabbit polyclonal antibody (STJ28429) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse pancreatic islet using Synaptophysin rabbit polyclonal antibody (STJ28429) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Synaptophysin rabbit polyclonal antibody (STJ28429) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Neuro-2a cells using Synaptophysin rabbit polyclonal antibody (STJ28429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of SH-SY5Y cells using Synaptophysin rabbit polyclonal antibody (STJ28429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat brain using Synaptophysin rabbit polyclonal antibody (STJ28429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human pancreas using Synaptophysin rabbit polyclonal antibody (STJ28429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain using Synaptophysin rabbit polyclonal antibody (STJ28429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 600 Mu g extracts of mouse brain cells using 3 Mu g Synaptophysin antibody (STJ28429). Western blot was performed from the immunoprecipitate using Synaptophysin antibody (STJ28429) at a dilution of 1:1000.

Anti-SYP antibody (222-313) (STJ28429)

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STJ28429

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SYP (222-313) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SYP
Gene ID: 6855
Uniprot ID: SYPH_HUMAN
Immunogen Region: 222-313
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 222-313 of human Synaptophysin (NP_003170.1).
Immunogen Sequence: VFKETGWAAPFLRAPPGAPE KQPAPGDAYGDAGYGQGPGG YGPQDSYGPQGGYQPDYGQP AGSGGSGYGPQGDYGQQGYG PQGAPTSFSNQM
Tissue Specificity Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3. Expressed in the putamen (at protein level).
Post Translational Modifications Ubiquitinated.mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. Phosphorylated by SRC.
Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
Protein Name Synaptophysin
Major Synaptic Vesicle Protein P38
Database Links Reactome: R-HSA-9662360
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Multi-Pass Membrane Protein
Synapse
Synaptosome
Alternative Antibody Names Anti-Synaptophysin antibody
Anti-Major Synaptic Vesicle Protein P38 antibody
Anti-SYP antibody

Information sourced from Uniprot.org

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