Confocal imaging of paraffin-embedded Rat brain using Synapsin II Rabbit monoclonal antibody (STJ11103035, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Synapsin II antibody (200-300) [S5MR] (STJ11103035)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103035
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General Information

Short DescriptionRabbit monoclonal Synapsin II (200-300) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS5MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:2000
IF/ICC:1:200-1:800
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSYN2
Gene ID6854
Uniprot IDSYN2_HUMAN
Immunogen Region200-300
Immunogen SequenceHLIIGMQYAGLPSINSLESI YNFCDKPWVFAQLVAIYKTL GGEKFPLIEQTYYPNHKEML TLPTFPVVVKIGHAHSGMGK VKVENHYDFQDIASVVALTQ T
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human Synapsin II (Q92777).

Additional Info

Tissue Specificity Central and peripheral nervous systems.
Post Translational Modifications Phosphorylation at Ser-10 dissociates synapsins from synaptic vesicles. Phosphorylation at Ser-425 by MAPK1/ERK2 and/or MAPK3/ERK1 may play a role in noradrenaline secretion by sympathetic neurons.
Function Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons.
Protein Name Synapsin-2
Synapsin Ii
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-212676
Cellular Localisation Synapse
Alternative Antibody Names Anti-Synapsin-2 antibody
Anti-Synapsin Ii antibody
Anti-SYN2 antibody

Information sourced from Uniprot.org

Citations

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