• Western blot analysis of extracts of various cell lines, using SYCP3 antibody (STJ111740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using SYCP3 Rabbit polyclonal antibody (STJ111740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SYCP3 antibody (1-236) (STJ111740)

SKU:
STJ111740

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SYCP3 (1-236) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SYCP3
Gene ID: 50511
Uniprot ID: SYCP3_HUMAN
Immunogen Region: 1-236
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of human SYCP3 (NP_710161.1).
Immunogen Sequence: MVSSGKKYSRKSGKPSVEDQ FTRAYDFETEDKKDLSGSEE DVIEGKTAVIEKRRKKRSSA GVVEDMGGEVQNMLEGVGVD INKALLAKRKRLEMYTKASL KTSNQKIEHVWKTQQDQRQK LNQEYSQQFLTLFQQWDLDM QKAEEQEEKILNMFRQQQKI LQQSRIVQSQRLKTIKQLYE QFIKSMEELEKNHDNLLTGA QNEFKKEMAMLQKKIMMETQ QQEIASVRKSLQSMLF
Tissue Specificity Testis-specific.
Post Translational Modifications Phosphorylated.
Function Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Required for centromere pairing during meiosis in male germ cells. Required for normal meiosis during spermatogenesis and male fertility. Plays a lesser role in female fertility. Required for efficient phosphorylation of HORMAD1 and HORMAD2.
Protein Name Synaptonemal Complex Protein 3
Scp-3
Database Links Reactome: R-HSA-1221632
Cellular Localisation Nucleus
Chromosome
Centromere
It Is Present In Early Unpaired Cores
In The Lateral Domains Of The Synaptonemal Complex And In The Chromosome Cores When They Separate At Diplotene
It Is Found Axial To The Metaphase I Chromosomes And In Association With Pairs Of Sister Centromeres
The Centromere-Associated Protein Becomes Dissociated From The Centromeres At Anaphase Ii And Is Not Found In Mitotic Metaphase Centromeres
Alternative Antibody Names Anti-Synaptonemal Complex Protein 3 antibody
Anti-Scp-3 antibody
Anti-SYCP3 antibody
Anti-SCP3 antibody

Information sourced from Uniprot.org

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