• Immunofluorescence analysis of L929 cells using SWAP70 Rabbit polyclonal antibody (STJ117057) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SWAP70 in paraffin-embedded Human lung using SWAP70 Rabbit polyclonal antibody (STJ117057) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SWAP70 in paraffin-embedded Rat spleen using SWAP70 Rabbit polyclonal antibody (STJ117057) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SWAP70 in paraffin-embedded Mouse spleen using SWAP70 Rabbit polyclonal antibody (STJ117057) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SWAP70 Rabbit polyclonal antibody (STJ117057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SWAP70 antibody (316-585) (STJ117057)

SKU:
STJ117057

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SWAP70 (316-585) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SWAP70
Gene ID: 23075
Uniprot ID: SWP70_HUMAN
Immunogen Region: 316-585
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 316-585 of human SWAP70 (NP_055870.2).
Immunogen Sequence: HKEARQRRKELRKKQLAEQE ELERQMKELQAANESKQQEL EAVRKKLEEAASRAAEEEKK RLQTQVELQARFSTELEREK LIRQQMEEQVAQKSSELEQY LQRVRELEDMYLKLQEALED ERQARQDEETVRKLQARLLE EESSKRAELEKWHLEQQQAI QTTEAEKQELENQRVLKEQA LQEAMEQLEQLELERKQALE QYEEVKKKLEMATNKTKSWK DKVAHHEGLIRLIEPGSKN
Tissue Specificity Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue. Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages.
Post Translational Modifications Tyrosine-phosphorylated.
Function Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
Protein Name Switch-Associated Protein 70
Swap-70
Database Links Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Cellular Localisation Cytoplasm
Cell Membrane
Nucleus
Cell Projection
Lamellipodium
Cytoskeleton
In Resting B-Cells It Is Localized Mainly In The Cytoplasm And Upon Cell Activation It Is Recruited To The Plasma Membrane And Then Translocates To The Nucleus
In Activated
Class-Switching B-Cells It Is Associated With Membrane Igg But Not Igm
Localized To Loose Actin Filament Arrays Located Behind Actively Extending Lamellipodia
Alternative Antibody Names Anti-Switch-Associated Protein 70 antibody
Anti-Swap-70 antibody
Anti-SWAP70 antibody
Anti-KIAA0640 antibody
Anti-HSPC321 antibody

Information sourced from Uniprot.org

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