| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SWAP70 |
| Gene ID: | 23075 |
| Uniprot ID: | SWP70_HUMAN |
| Immunogen Region: | 316-585 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 316-585 of human SWAP70 (NP_055870.2). |
| Immunogen Sequence: | HKEARQRRKELRKKQLAEQE ELERQMKELQAANESKQQEL EAVRKKLEEAASRAAEEEKK RLQTQVELQARFSTELEREK LIRQQMEEQVAQKSSELEQY LQRVRELEDMYLKLQEALED ERQARQDEETVRKLQARLLE EESSKRAELEKWHLEQQQAI QTTEAEKQELENQRVLKEQA LQEAMEQLEQLELERKQALE QYEEVKKKLEMATNKTKSWK DKVAHHEGLIRLIEPGSKN |
| Tissue Specificity | Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue. Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages. |
| Post Translational Modifications | Tyrosine-phosphorylated. |
| Function | Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. |
| Protein Name | Switch-Associated Protein 70 Swap-70 |
| Database Links | Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013423 |
| Cellular Localisation | Cytoplasm Cell Membrane Nucleus Cell Projection Lamellipodium Cytoskeleton In Resting B-Cells It Is Localized Mainly In The Cytoplasm And Upon Cell Activation It Is Recruited To The Plasma Membrane And Then Translocates To The Nucleus In Activated Class-Switching B-Cells It Is Associated With Membrane Igg But Not Igm Localized To Loose Actin Filament Arrays Located Behind Actively Extending Lamellipodia |
| Alternative Antibody Names | Anti-Switch-Associated Protein 70 antibody Anti-Swap-70 antibody Anti-SWAP70 antibody Anti-KIAA0640 antibody Anti-HSPC321 antibody |
Information sourced from Uniprot.org