Anti-SUPT16H antibody (520-695) (STJ11101487)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SUPT16H (SPT16) (520-695) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SUPT16H |
| Gene ID | 11198 |
| Uniprot ID | SP16H_HUMAN |
| Immunogen Region | 520-695 |
| Immunogen Sequence | KEPHIREMKIYIDKKYETVI MPVFGIATPFHIATIKNISM SVEGDYTYLRINFYCPGSAL GRNEGNIFPNPEATFVKEIT YRASNIKAPGEQTVPALNLQ NAFRIIKEVQKRYKTREAEE KEKEGIVKQDSLVINLNRSN PKLKDLYIRPNIAQKRMQGS LEAHVNGFRFTSVRGD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 520-695 of human SUPT16H (SPT16) (NP_009123.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin. |
| Function | Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). |
| Protein Name | Fact Complex Subunit Spt16Chromatin-Specific Transcription Elongation Factor 140 Kda SubunitFact 140 Kda SubunitFactp140Facilitates Chromatin Transcription Complex Subunit Spt16Hspt16 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-167152Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-6804756Reactome: R-HSA-75955 |
| Cellular Localisation | NucleusChromosomeColocalizes With Rna Polymerase Ii On ChromatinRecruited To Actively Transcribed Loci |
| Alternative Antibody Names | Anti-Fact Complex Subunit Spt16 antibodyAnti-Chromatin-Specific Transcription Elongation Factor 140 Kda Subunit antibodyAnti-Fact 140 Kda Subunit antibodyAnti-Factp140 antibodyAnti-Facilitates Chromatin Transcription Complex Subunit Spt16 antibodyAnti-Hspt16 antibodyAnti-SUPT16H antibodyAnti-FACT140 antibodyAnti-FACTP140 antibody |
Information sourced from Uniprot.org