• Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night
  • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SUMO2 antibody (45-95 aa) (STJ196508)

SKU:
STJ196508

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Small ubiquitin-related modifier 2 (45-95 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUMO2
Gene ID: 6613
Uniprot ID: SUMO2_HUMAN
Immunogen Region: 45-95 aa
Specificity: This antibody detects endogenous levels of human SUMO2
Immunogen: Synthesized peptide derived from the human SUMO2 at the amino acid range 45-95
Post Translational Modifications Polymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4. Cleavage of precursor form by SENP1 or SENP2 is necessary for function. Monoubiquitinated N-terminally by UBE2W, which primes it for RNF4-dependent polyubiquitination by the UBE2V1-UBE2N heterodimer.
Function Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Plays a role in the regulation of sumoylation status of SETX.
Protein Name Small Ubiquitin-Related Modifier 2
Sumo-2
Hsmt3
Smt3 Homolog 2
Sumo-3
Sentrin-2
Ubiquitin-Like Protein Smt3b
Smt3b
Database Links Reactome: R-HSA-196791
Reactome: R-HSA-3065676
Reactome: R-HSA-3065678
Reactome: R-HSA-3065679
Reactome: R-HSA-3108214
Reactome: R-HSA-3232118
Reactome: R-HSA-3899300
Reactome: R-HSA-4085377
Reactome: R-HSA-4090294
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4615885
Reactome: R-HSA-5693607
Reactome: R-HSA-5696395
Cellular Localisation Nucleus
Pml Body
Alternative Antibody Names Anti-Small Ubiquitin-Related Modifier 2 antibody
Anti-Sumo-2 antibody
Anti-Hsmt3 antibody
Anti-Smt3 Homolog 2 antibody
Anti-Sumo-3 antibody
Anti-Sentrin-2 antibody
Anti-Ubiquitin-Like Protein Smt3b antibody
Anti-Smt3b antibody
Anti-SUMO2 antibody
Anti-SMT3B antibody
Anti-SMT3H2 antibody

Information sourced from Uniprot.org

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