• Immunofluorescence analysis of L929 cells using SUMF2 Rabbit polyclonal antibody (STJ119720) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SUMF2 Rabbit polyclonal antibody (STJ119720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SUMF2 antibody (45-320) (STJ119720)

SKU:
STJ119720

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUMF2 (45-320) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUMF2
Gene ID: 25870
Uniprot ID: SUMF2_HUMAN
Immunogen Region: 45-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 45-320 of human SUMF2 (NP_056226.2).
Immunogen Sequence: QATSMVQLQGGRFLMGTNSP DSRDGEGPVREATVKPFAID IFPVTNKDFRDFVREKKYRT EAEMFGWSFVFEDFVSDELR NKATQPMKSVLWWLPVEKAF WRQPAGPGSGIRERLEHPVL HVSWNDARAYCAWRGKRLPT EEEWEFAARGGLKGQVYPWG NWFQPNRTNLWQGKFPKGDK AEDGFHGVSPVNAFPAQNNY GLYDLLGNVWEWTASPYQAA EQDMRVLRGASWIDTADGS
Tissue Specificity Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in kidney, liver and placenta.
Function Lacks formylglycine generating activity and is unable to convert newly synthesized inactive sulfatases to their active form. Inhibits the activation of sulfatases by SUMF1.
Protein Name Inactive C-Alpha-Formylglycine-Generating Enzyme 2
Paralog Of Formylglycine-Generating Enzyme
Pfge
Sulfatase-Modifying Factor 2
Database Links Reactome: R-HSA-1660662
Reactome: R-HSA-1663150
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Inactive C-Alpha-Formylglycine-Generating Enzyme 2 antibody
Anti-Paralog Of Formylglycine-Generating Enzyme antibody
Anti-Pfge antibody
Anti-Sulfatase-Modifying Factor 2 antibody
Anti-SUMF2 antibody
Anti-PSEC0171 antibody
Anti-UNQ1968 antibody
Anti-PRO4500 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance