| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | STRADA |
| Gene ID: | 92335 |
| Uniprot ID: | STRAA_HUMAN |
| Immunogen Region: | 25-210 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human STRADA (NP_699166.2). |
| Immunogen Sequence: | FLPEGGCYELLTVIGKGFED LMTVNLARYKPTGEYVTVRR INLEACSNEMVTFLQGELHV SKLFNHPNIVPYRATFIADN ELWVVTSFMAYGSAKDLICT HFMDGMNELAIAYILQGVLK ALDYIHHMGYVHRSVKASHI LISVDGKVYLSGLRSNLSMI SHGQRQRVVHDFPKYSVKVL PWLSPE |
| Function | Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation. |
| Protein Name | Ste20-Related Kinase Adapter Protein AlphaStrad AlphaSte20-Related Adapter ProteinSerologically Defined Breast Cancer Antigen Ny-Br-96 |
| Database Links | Reactome: R-HSA-380972 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Ste20-Related Kinase Adapter Protein Alpha antibodyAnti-Strad Alpha antibodyAnti-Ste20-Related Adapter Protein antibodyAnti-Serologically Defined Breast Cancer Antigen Ny-Br-96 antibodyAnti-STRADA antibodyAnti-LYK5 antibodyAnti-STRAD antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and STRADA knockout (KO) HeLa cells, using [KO Validated] STRADA Rabbit polyclonal antibody (STJ119985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98770/382819/STJ119985_1__92297.1713151316.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and STRADA knockout (KO) HeLa cells, using [KO Validated] STRADA Rabbit polyclonal antibody (STJ119985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
