• Immunofluorescence analysis of NIH/3T3 cells using STK33 Rabbit monoclonal antibody (STJ11103145) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of SH-SY5Y cells, using STK33 Rabbit monoclonal antibody (STJ11103145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-STK33 antibody (399-514) [S5MR] (STJ11103145)

SKU:
STJ11103145

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-STK33 (399-514) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STK33
Gene ID: 65975
Uniprot ID: STK33_HUMAN
Immunogen Region: 399-514
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 399-514 of human STK33 (Q9BYT3).
Immunogen Sequence: KEWKNNPESVEENTTEEKNK PSTEEKLKSYQPWGNVPDAN YTSDEEEEKQSTAYEKQFPA TSKDNFDMCSSSFTSSKLLP AEIKGEMEKTPVTPSQGTAT KYPAKSGALSRTKKKL
Tissue Specificity Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.
Post Translational Modifications Autophosphorylated.
Function Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME. Not essential for the survival of KRAS-dependent AML cell lines.
Protein Name Serine/Threonine-Protein Kinase 33
Cellular Localisation Cytoplasm
Perinuclear Region
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase 33 antibody
Anti-STK33 antibody

Information sourced from Uniprot.org

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