Anti-STIM1 antibody (N-Term) (STJA0010329)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human N-ternal STIM1
STJA0010329
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General Information

Short DescriptionRabbit polyclonal anti-Stromal interaction molecule 1 (N-Term) for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC/WB
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
IHC 1:50-200
FormulationLiquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSTIM1
Gene ID6786
Uniprot IDSTIM1_HUMAN
ImmunogenSynthesized peptide derived from human N-ternal STIM1
Immunogen RegionN-Term
SpecificityThis antibody detects endogenous levels of STIM1 at Human, Mouse, Rat

Additional Info

Post Translational Modifications Glycosylation is required for cell surface expression. Phosphorylated predominantly on Ser residues.
Function Acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels. Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry. Involved in enamel formation.
Protein Name Stromal Interaction Molecule 1
Database Links Reactome: R-HSA-139853
Reactome: R-HSA-5578775
Reactome: R-HSA-983695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum Membrane
Cytoplasm
Cytoskeleton
Sarcoplasmic Reticulum
Translocates From The Endoplasmic Reticulum To The Cell Membrane In Response To A Depletion Of Intracellular Calcium And Is Detected At Punctae Corresponding To Junctions Between The Endoplasmic Reticulum And The Cell Membrane
Associated With The Microtubule Network At The Growing Distal Tip Of Microtubules
Colocalizes With Orai1 At The Cell Membrane
Colocalizes Preferentially With Casq1 At Endoplasmic Reticulum In Response To A Depletion Of Intracellular Calcium
Alternative Antibody Names Anti-Stromal Interaction Molecule 1 antibody
Anti-STIM1 antibody
Anti-GOK antibody

Information sourced from Uniprot.org

Citations

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