| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/FC |
| Reactivity: |
Human/Mouse/Rat/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-STIM1 (Internal) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:32000.WB: Successful use of this antibody was reported in Western blot showing an approx. 80kDa in Human platelets: PMID 18439569FC:Flow cytometric analysis of HepG2 cells. Recommended conc |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
STIM1 |
| Gene ID: |
6786 |
| Uniprot ID: |
STIM1_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_003147.2; NP_001264890.1; NP_001264891.1 |
| Immunogen Sequence: |
YIQNRYSKEHMKK |
| Post Translational Modifications | Glycosylation is required for cell surface expression. Phosphorylated predominantly on Ser residues. |
| Function | Acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels. Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry. Involved in enamel formation. |
| Protein Name | Stromal Interaction Molecule 1 |
| Database Links | Reactome: R-HSA-139853Reactome: R-HSA-5578775Reactome: R-HSA-983695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum MembraneCytoplasmCytoskeletonSarcoplasmic ReticulumTranslocates From The Endoplasmic Reticulum To The Cell Membrane In Response To A Depletion Of Intracellular Calcium And Is Detected At Punctae Corresponding To Junctions Between The Endoplasmic Reticulum And The Cell MembraneAssociated With The Microtubule Network At The Growing Distal Tip Of MicrotubulesColocalizes With Orai1 At The Cell MembraneColocalizes Preferentially With Casq1 At Endoplasmic Reticulum In Response To A Depletion Of Intracellular Calcium |
| Alternative Antibody Names | Anti-Stromal Interaction Molecule 1 antibodyAnti-STIM1 antibodyAnti-GOK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and STIM1 knockout (KO) HeLa cells, using [KO Validated] STIM1 Rabbit polyclonal antibody (STJ11100770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89707/358189/STJ11100770_1__47615.1713122504.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and STIM1 knockout (KO) HeLa cells, using [KO Validated] STIM1 Rabbit polyclonal antibody (STJ11100770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
