![Western blot analysis of various lysates using [KO Validated] STAT5B Rabbit monoclonal antibody (STJ11101702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90617/360470/STJ11101702_2__75059.1713125131.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] STAT5B Rabbit monoclonal antibody (STJ11101702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and STAT5B knockout (KO) HeLa cells, using [KO Validated] STAT5B Rabbit monoclonal antibody (STJ11101702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90617/360471/STJ11101702_3__75885.1713125132.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and STAT5B knockout (KO) HeLa cells, using [KO Validated] STAT5B Rabbit monoclonal antibody (STJ11101702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-STAT5B antibody (600-787) [S2MR] (STJ11101702)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal STAT5B (600-787) antibody for use in WB, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S2MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | STAT5B |
| Gene ID | 6777 |
| Uniprot ID | STA5B_HUMAN |
| Immunogen Region | 600-787 |
| Immunogen Sequence | KQQAHDLLINKPDGTFLLRF SDSEIGGITIAWKFDSQERM FWNLMPFTTRDFSIRSLADR LGDLNYLIYVFPDRPKDEVY SKYYTPVPCESATAKAVDGY VKPQIKQVVPEFVNASADAG GGSATYMDQAPSPAVCPQAH YNMYPQNPDSVLDTDGDFDL EDTMDVARRVEELLGRPMDS QWIPHAQS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 600-787 of human STAT5B (P51692). |
Additional Info
| Post Translational Modifications | Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3.wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. |
| Function | Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation. |
| Protein Name | Signal Transducer And Activator Of Transcription 5b |
| Database Links | Reactome: R-HSA-1170546Reactome: R-HSA-1266695Reactome: R-HSA-1433557Reactome: R-HSA-1839117Reactome: R-HSA-186763Reactome: R-HSA-2586552Reactome: R-HSA-512988Reactome: R-HSA-8854691Reactome: R-HSA-8983432Reactome: R-HSA-8985947Reactome: R-HSA-9020558Reactome: R-HSA-9020958Reactome: R-HSA-9027283Reactome: R-HSA-9645135Reactome: R-HSA-9670439Reactome: R-HSA-9674555Reactome: R-HSA-9702518Reactome: R-HSA-9703465Reactome: R-HSA-9705462Reactome: R-HSA-982772 |
| Cellular Localisation | CytoplasmNucleusTranslocated Into The Nucleus In Response To Phosphorylation |
| Alternative Antibody Names | Anti-Signal Transducer And Activator Of Transcription 5b antibodyAnti-STAT5B antibody |
Information sourced from Uniprot.org