| Short Description : | Mouse monoclonal anti-Stat5 (C-Term) for use in ELISA, WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | ELISA/WB/ICC |
| Host: | Mouse |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | M251 |
| Isotype: | IgG1 |
| Conjugation: | Unconjugated |
| Purification: | Protein A Purified |
| Dilution Range: | WB 1:1000ICC 1:100 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction: | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
| Gene Symbol: | STAT5A |
| Gene ID: | 6776 |
| Uniprot ID: | STA5A_HUMAN |
| Immunogen: | Clone (M251) was generated from a recombinant protein that included amino acid residues in the C-terminal region of human Stat5A. This sequence has high homology to the conserved regions in rat and mouse Stat5A and B. |
| Immunogen Region: | C-Term |
| Immunogen Sequence: | Human |
| Specificity: | The antibody detects a 92 kDa* band corresponding to Stat5A on SDS-PAGE immunoblots of human A431 cells. |
| Post Translational Modifications | Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. Tyrosine phosphorylated in response to signaling via activated FLT3.wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694. ISGylated. |
| Function | Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. |
| Protein Name | Signal Transducer And Activator Of Transcription 5a |
| Database Links | Reactome: R-HSA-1170546Reactome: R-HSA-1251985Reactome: R-HSA-1266695Reactome: R-HSA-1433557Reactome: R-HSA-1839117Reactome: R-HSA-186763Reactome: R-HSA-2586552Reactome: R-HSA-512988Reactome: R-HSA-8854691Reactome: R-HSA-8983432Reactome: R-HSA-8985947Reactome: R-HSA-9020558Reactome: R-HSA-9020958Reactome: R-HSA-9027283Reactome: R-HSA-9645135Reactome: R-HSA-9670439Reactome: R-HSA-9674555Reactome: R-HSA-9702518Reactome: R-HSA-9703465Reactome: R-HSA-9705462Reactome: R-HSA-9725371Reactome: R-HSA-982772 |
| Cellular Localisation | CytoplasmNucleusTranslocated Into The Nucleus In Response To Phosphorylation |
| Alternative Antibody Names | Anti-Signal Transducer And Activator Of Transcription 5a antibodyAnti-STAT5A antibodyAnti-STAT5 antibody |
Information sourced from Uniprot.org
