Anti-STARD3 antibody (STJ193076)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
General Information
| Short Description | Rabbit polyclonal anti-StAR-related lipid transfer protein 3 for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | STARD3 |
| Gene ID | 10948 |
| Uniprot ID | STAR3_HUMAN |
| Immunogen | Synthesized peptide derived from part of the human protein |
| Specificity | STAR3 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-209 is necessary and sufficient for the direct interaction of the phosphorylated FFAT motif with the MSP domain of MOSPD2, VAPA and VAPB and allows the tethering of two membranes that participates in the formation of ER-endosome contacts. Phosphorylation of the FFAT motif leads to conformation changes. Additional phosphorylations around the core FFAT motif (QFYSPPE) are not essential but strengthen the interaction with MOSPD2, VAPA and VAPB. Phosphorylation at Ser-209 of FFAT motif drives membrane tethering between the endoplasmic reticulum and late endosomes via interaction with VAPA and VAPB that in turn allows the efficient transport of sterol mediated by the START domain. |
| Function | Sterol-binding protein that mediates cholesterol transport from the endoplasmic reticulum to endosomes. The sterol transport mechanism is triggered by phosphorylation of FFAT motif that leads to membrane tethering between the endoplasmic reticulum and late endosomes via interaction with VAPA and VAPB. Acts as a lipid transfer protein that redirects sterol to the endosome at the expense of the cell membrane and favors membrane formation inside endosomes. May also mediate cholesterol transport between other membranes, such as mitochondria membrane or cell membrane. However, such results need additional experimental evidences.probably mainly mediates cholesterol transport from the endoplasmic reticulum to endosomes. Does not activate transcriptional cholesterol sensing. Able to bind other lipids, such as lutein, a xanthophyll carotenoids that form the macular pigment of the retina. |
| Protein Name | Star-Related Lipid Transfer Protein 3Metastatic Lymph Node Gene 64 ProteinMln 64Protein Cab1Start Domain-Containing Protein 3Stard3 |
| Database Links | Reactome: R-HSA-196108 |
| Cellular Localisation | Late Endosome MembraneMulti-Pass Membrane ProteinLocalizes To Contact Sites Between The Endoplasmic Reticulum And Late Endosomes: Associates With The Endoplasmic Reticulum Membrane Via Interaction With VapaVapb Or Mospd2 |
| Alternative Antibody Names | Anti-Star-Related Lipid Transfer Protein 3 antibodyAnti-Metastatic Lymph Node Gene 64 Protein antibodyAnti-Mln 64 antibodyAnti-Protein Cab1 antibodyAnti-Start Domain-Containing Protein 3 antibodyAnti-Stard3 antibodyAnti-STARD3 antibodyAnti-CAB1 antibodyAnti-MLN64 antibody |
Information sourced from Uniprot.org