• Immunohistochemistry analysis of ST6GAL1 in paraffin-embedded mouse lung using ST6GAL1 Rabbit polyclonal antibody (STJ28321) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ST6GAL1 in paraffin-embedded human stomach using ST6GAL1 Rabbit polyclonal antibody (STJ28321) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Daudi cells, using ST6GAL1 Rabbit polyclonal antibody (STJ28321) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ST6GAL1 antibody (27-200) (STJ28321)

SKU:
STJ28321

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ST6GAL1 (27-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ST6GAL1
Gene ID: 6480
Uniprot ID: SIAT1_HUMAN
Immunogen Region: 27-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-200 of human ST6GAL1 (NP_775323.1).
Immunogen Sequence: KEKKKGSYYDSFKLQTKEFQ VLKSLGKLAMGSDSQSVSSS STQDPHRGRQTLGSLRGLAK AKPEASFQVWNKDSSSKNLI PRLQKIWKNYLSMNKYKVSY KGPGPGIKFSAEALRCHLRD HVNVSMVEVTDFPFNTSEWE GYLPKESIRTKAGPWGRCAV VSSAGSLKSSQLGR
Post Translational Modifications The soluble form derives from the membrane form by proteolytic processing. The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme. N-glycosylated.
Function Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.
Protein Name Beta-Galactoside Alpha-2 -6-Sialyltransferase 1
Alpha 2 -6-St 1
B-Cell Antigen Cd75
Cmp-N-Acetylneuraminate-Beta-Galactosamide-Alpha-2 -6-Sialyltransferase 1
St6gal I
St6gali
Sialyltransferase 1
Database Links Reactome: R-HSA-4085001
Reactome: R-HSA-9683673
Reactome: R-HSA-9694548
Reactome: R-HSA-9694719
Reactome: R-HSA-975577
Reactome: R-HSA-977068
Cellular Localisation Golgi Apparatus
Golgi Stack Membrane
Single-Pass Type Ii Membrane Protein
Secreted
Membrane-Bound Form In Trans Cisternae Of Golgi
Secreted Into The Body Fluid
Alternative Antibody Names Anti-Beta-Galactoside Alpha-2 -6-Sialyltransferase 1 antibody
Anti-Alpha 2 -6-St 1 antibody
Anti-B-Cell Antigen Cd75 antibody
Anti-Cmp-N-Acetylneuraminate-Beta-Galactosamide-Alpha-2 -6-Sialyltransferase 1 antibody
Anti-St6gal I antibody
Anti-St6gali antibody
Anti-Sialyltransferase 1 antibody
Anti-ST6GAL1 antibody
Anti-SIAT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance