• Western blot analysis of lysates from A549 cells, using Somatostatin Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from 1) PC-12, 2) A549, 3) Hela cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • Immunohistochemical analysis of paraffin-embedded Human Gastric fundus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Somatostatin Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human Gastric fundus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Gastric fundus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of A549 cells using Somatostatin Polyclonal Antibody
  • Western blot analysis of various cells using Somatostatin Polyclonal Antibody
  • Western blot analysis of lysates from 1) PC-12, 2) A549, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-SST antibody (10-59 aa) (STJ95730)

SKU:
STJ95730

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Somatostatin (10-59 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SST
Gene ID: 6750
Uniprot ID: SMS_HUMAN
Immunogen Region: 10-59 aa
Specificity: Somatostatin Polyclonal Antibody detects endogenous levels of Somatostatin protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Somatostatin at the amino acid range 10-59
Function Somatostatin-14: Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH.the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin. Neuronostatin: May enhance low-glucose-induced glucagon release by pancreatic alpha cells. This effect may be mediated by binding to GPR107 and PKA activation. May regulate cardiac contractile function. May compromise cardiomyocyte viability. In the central nervous system, may impair memory retention and may affect hippocampal excitability. May also have anxiolytic and anorexigenic effects. May play a role in arterial pressure regulation. May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH.
Protein Name Somatostatin
Growth Hormone Release-Inhibiting Factor Cleaved Into - Somatostatin-28 - Somatostatin-14
Sst-14 - Neuronostatin
Nst
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Reactome: R-HSA-9022702
Cellular Localisation Secreted
Alternative Antibody Names Anti-Somatostatin antibody
Anti-Growth Hormone Release-Inhibiting Factor Cleaved Into - Somatostatin-28 - Somatostatin-14 antibody
Anti-Sst-14 - Neuronostatin antibody
Anti-Nst antibody
Anti-SST antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance