• Immunofluorescence analysis of U-2 OS cells using SRSF3 Rabbit polyclonal antibody (STJ27861) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using SRSF3 Rabbit polyclonal antibody (STJ27861) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SRSF3 Rabbit polyclonal antibody (STJ27861) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SRSF3 in paraffin-embedded Mouse testis using SRSF3 Rabbit polyclonal antibody (STJ27861) at dilution of 1:100 (20x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SRSF3 in paraffin-embedded Human mammary cancer using SRSF3 Rabbit polyclonal antibody (STJ27861) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SRSF3 in paraffin-embedded Rat brain using SRSF3 Rabbit polyclonal antibody (STJ27861) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SRSF3 Rabbit polyclonal antibody (STJ27861) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SRSF3 antibody (1-90) (STJ27861)

SKU:
STJ27861

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:100-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRSF3
Gene ID: 6428
Uniprot ID: SRSF3_HUMAN
Immunogen Region: 1-90
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human SRSF3 (NP_003008.1).
Immunogen Sequence: MHRDSCPLDCKVYVGNLGNN GNKTELERAFGYYGPLRSVW VARNPPGFAFVEFEDPRDAA DAVRELDGRTLCGCRVRVEL SNGEKRSRNR
Post Translational Modifications Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain.
Function Splicing factor, which binds the consensus motif 5'-CACUAUCACUACC-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons. Also functions as an adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway).enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.
Protein Name Serine/Arginine-Rich Splicing Factor 3
Pre-Mrna-Splicing Factor Srp20
Splicing Factor - Arginine/Serine-Rich 3
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72163
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Cellular Localisation Nucleus
Nucleus Speckle
Cytoplasm
Recruited To Nuclear Speckles Following Interaction With Ythdc1
Alternative Antibody Names Anti-Serine/Arginine-Rich Splicing Factor 3 antibody
Anti-Pre-Mrna-Splicing Factor Srp20 antibody
Anti-Splicing Factor - Arginine/Serine-Rich 3 antibody
Anti-SRSF3 antibody
Anti-SFRS3 antibody
Anti-SRP20 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance