| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SRSF10 (163-262) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SRSF10 |
| Gene ID: |
10772 |
| Uniprot ID: |
SRS10_HUMAN |
| Immunogen Region: |
163-262 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 163-262 of human SRSF10 (NP_473357.1). |
| Immunogen Sequence: |
DRFKHRNRSFSRSKSNSRSR SKSQPKKEMKAKSRSRSASH TKTRGTSKTDSKTHYKSGSR YEKESRKKEPPRSKSQSRSQ SRSRSKSRSRSWTSPKSSGH |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylated. Fully dephosphorylated in mitosis and partially dephosphorylated on heat shock. |
| Function | Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70. Required for splicing repression in M-phase cells and after heat shock. Also acts as a splicing factor that specifically promotes exon skipping during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator. |
| Protein Name | Serine/Arginine-Rich Splicing Factor 1040 Kda Sr-Repressor ProteinSrrp40Fus-Interacting Serine-Arginine-Rich Protein 1Splicing Factor Srp38Splicing Factor - Arginine/Serine-Rich 13aTls-Associated Protein With Ser-Arg RepeatsTasrTls-Associated Protein With Sr RepeatsTls-Associated Serine-Arginine ProteinTls-Associated Sr Protein |
| Database Links | Reactome: R-HSA-72163Reactome: R-HSA-72203 |
| Cellular Localisation | Nucleus SpeckleCytoplasm |
| Alternative Antibody Names | Anti-Serine/Arginine-Rich Splicing Factor 10 antibodyAnti-40 Kda Sr-Repressor Protein antibodyAnti-Srrp40 antibodyAnti-Fus-Interacting Serine-Arginine-Rich Protein 1 antibodyAnti-Splicing Factor Srp38 antibodyAnti-Splicing Factor - Arginine/Serine-Rich 13a antibodyAnti-Tls-Associated Protein With Ser-Arg Repeats antibodyAnti-Tasr antibodyAnti-Tls-Associated Protein With Sr Repeats antibodyAnti-Tls-Associated Serine-Arginine Protein antibodyAnti-Tls-Associated Sr Protein antibodyAnti-SRSF10 antibodyAnti-FUSIP1 antibodyAnti-FUSIP2 antibodyAnti-SFRS13A antibodyAnti-TASR antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PSMB8 knockout (KO) A-549 cells, using [KO Validated] PSMB8 Rabbit polyclonal antibody (STJ11100809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89745/358219/STJ11100809_1__76245.1713122545.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PSMB8 knockout (KO) A-549 cells, using [KO Validated] PSMB8 Rabbit polyclonal antibody (STJ11100809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")