• Immunofluorescence analysis of U-2 OS cells using SRP54 Rabbit monoclonal antibody (STJ11103481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using SRP54 Rabbit monoclonal antibody (STJ11103481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SRP54 Rabbit monoclonal antibody (STJ11103481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using SRP54 antibody (STJ11103481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SRP54 antibody (400-504) [S1MR] (STJ11103481)

SKU:
STJ11103481

£146.50 - £639.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SRP54 (400-504) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRP54
Gene ID: 6729
Uniprot ID: SRP54_HUMAN
Immunogen Region: 400-504
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-504 of human SRP54 (P61011).
Immunogen Sequence: PGRIQRVARGSGVSTRDVQE LLTQYTKFAQMVKKMGGIKG LFKGGDMSKNVSQSQMAKLN QQMAKMMDPRVLHHMGGMAG LQSMMRQFQQGAAGNMKGMM GFNNM
Function Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). As part of the SRP complex, associates with the SRP receptor (SR) component SRPRA to target secretory proteins to the endoplasmic reticulum membrane. Binds to the signal sequence of presecretory proteins when they emerge from the ribosomes. Displays basal GTPase activity, and stimulates reciprocal GTPase activation of the SR subunit SRPRA. Forms a guanosine 5'-triphosphate (GTP)-dependent complex with the SR subunit SRPRA. SR compaction and GTPase mediated rearrangement of SR drive SRP-mediated cotranslational protein translocation into the ER. Requires the presence of SRP9/SRP14 and/or SRP19 to stably interact with RNA. Plays a role in proliferation and differentiation of granulocytic cells, neutrophils migration capacity and exocrine pancreas development.
Protein Name Signal Recognition Particle Subunit Srp54
Signal Recognition Particle 54 Kda Protein
Database Links Reactome: R-HSA-1799339
Cellular Localisation Nucleus Speckle
Cytoplasm
Endoplasmic Reticulum
Alternative Antibody Names Anti-Signal Recognition Particle Subunit Srp54 antibody
Anti-Signal Recognition Particle 54 Kda Protein antibody
Anti-SRP54 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance