| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
SREBF2 |
| Gene ID: |
6721 |
| Uniprot ID: |
SRBP2_HUMAN |
| Immunogen Region: |
401-500 aa |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 401-500 of human SREBF2 (NP_004590.2). |
| Immunogen Sequence: |
NKLLKGIDLGSLVDNEVDLK IEDFNQNVLLMSPPASDSGS QAGFSPYSIDSEPGSPLLDD AKVKDEPDSPPVALGMVDRS RILLCVLTFLCLSFNPLTSL |
| Tissue Specificity | Ubiquitously expressed in adult and fetal tissues. |
| Post Translational Modifications | Sterol regulatory element-binding protein 2: Processed in the Golgi apparatus, releasing the protein from the membrane. At low cholesterol the SCAP-SREBP complex is recruited into COPII vesicles for export from the endoplasmic reticulum. In the Golgi, complex SREBPs are cleaved sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain, releasing the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. Cleavage and activation is induced by mediated cholesterol efflux. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Processed sterol regulatory element-binding protein 2: Ubiquitinated.the nuclear form has a rapid turnover and is rapidly ubiquitinated and degraded by the proteasome in the nucleus. |
| Function | Sterol regulatory element-binding protein 2: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis. Processed sterol regulatory element-binding protein 2: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates transcription of genes related to cholesterol synthesis pathway. |
| Protein Name | Sterol Regulatory Element-Binding Protein 2Srebp-2Class D Basic Helix-Loop-Helix Protein 2Bhlhd2Sterol Regulatory Element-Binding Transcription Factor 2 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 2Transcription Factor Srebf2 |
| Database Links | Reactome: R-HSA-1655829Reactome: R-HSA-191273Reactome: R-HSA-1989781Reactome: R-HSA-2426168Reactome: R-HSA-381340Reactome: R-HSA-9619665 |
| Cellular Localisation | Sterol Regulatory Element-Binding Protein 2: Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneCytoplasmic VesicleCopii-Coated Vesicle MembraneAt High Sterol ConcentrationsThe Scap-Srebp Is Retained In The Endoplasmic ReticulumLow Sterol Concentrations Promote Recruitment Into Copii-Coated Vesicles And Transport Of The Scap-Srebp To The GolgiWhere It Is ProcessedProcessed Sterol Regulatory Element-Binding Protein 2: NucleusTransported Into The Nucleus With The Help Of Importin-BetaDimerization Of The Bhlh Domain Is A Prerequisite For Importin Beta-Dependent Nuclear Import |
| Alternative Antibody Names | Anti-Sterol Regulatory Element-Binding Protein 2 antibodyAnti-Srebp-2 antibodyAnti-Class D Basic Helix-Loop-Helix Protein 2 antibodyAnti-Bhlhd2 antibodyAnti-Sterol Regulatory Element-Binding Transcription Factor 2 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 2 antibodyAnti-Transcription Factor Srebf2 antibodyAnti-SREBF2 antibodyAnti-BHLHD2 antibodyAnti-SREBP2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
