• Immunofluorescence analysis of U-2 OS cells using Alpha Fodrin Rabbit polyclonal antibody (STJ114871) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Alpha Fodrin in paraffin-embedded mouse kidney using Alpha Fodrin Rabbit polyclonal antibody (STJ114871) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Alpha Fodrin in paraffin-embedded human stomach using Alpha Fodrin Rabbit polyclonal antibody (STJ114871) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Alpha Fodrin in paraffin-embedded rat liver using Alpha Fodrin Rabbit polyclonal antibody (STJ114871) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Alpha Fodrin Rabbit polyclonal antibody (STJ114871) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SPTAN1 antibody (950-1130) (STJ114871)

SKU:
STJ114871

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPTAN1 (950-1130) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPTAN1
Gene ID: 6709
Uniprot ID: SPTN1_HUMAN
Immunogen Region: 950-1130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 950-1130 of human Alpha Fodrin (NP_001123910.1).
Immunogen Sequence: REQAQSCRQQVAPTDDETGK ELVLALYDYQEKSPREVTMK KGDILTLLNSTNKDWWKVEV NDRQGFVPAAYVKKLDPAQS ASRENLLEEQGSIALRQEQI DNQTRITKEAGSVSLRMKQV EELYHSLLELGEKRKGMLEK SCKKFMLFREANELQQWINE KEAALTSEEVGADLEQVEVL Q
Post Translational Modifications Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
Function Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Protein Name Spectrin Alpha Chain - Non-Erythrocytic 1
Alpha-Ii Spectrin
Fodrin Alpha Chain
Spectrin - Non-Erythroid Alpha Subunit
Database Links Reactome: R-HSA-264870
Reactome: R-HSA-373753
Reactome: R-HSA-375165
Reactome: R-HSA-445095
Reactome: R-HSA-5673001
Reactome: R-HSA-6798695
Reactome: R-HSA-6807878
Reactome: R-HSA-9013420
Reactome: R-HSA-9013424
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Expressed Along The Cell Membrane In Podocytes And Presumptive Tubule Cells During Glomerulogenesis And Is Expressed Along Lateral Cell Margins In Tubule Cells
Alternative Antibody Names Anti-Spectrin Alpha Chain - Non-Erythrocytic 1 antibody
Anti-Alpha-Ii Spectrin antibody
Anti-Fodrin Alpha Chain antibody
Anti-Spectrin - Non-Erythroid Alpha Subunit antibody
Anti-SPTAN1 antibody
Anti-NEAS antibody
Anti-SPTA2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance