• Western blot analysis of extracts of various cell lines, using SPRR3 antibody (STJ113942) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of L929 cells using SPRR3 rabbit polyclonal antibody (STJ113942) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 using SPRR3 antibody (STJ113942) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS using SPRR3 antibody (STJ113942) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SPRR3 antibody (1-70) (STJ113942)

SKU:
STJ113942

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPRR3 (1-70) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPRR3
Gene ID: 6707
Uniprot ID: SPRR3_HUMAN
Immunogen Region: 1-70
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human SPRR3 (NP_005407.1).
Immunogen Sequence: MSSYQQKQTFTPPPQLQQQQ VKQPSQPPPQEIFVPTTKEP CHSKVPQPGNTKIPEPGCTK VPEPGCTKVP
Function Cross-linked envelope protein of keratinocytes.
Protein Name Small Proline-Rich Protein 3
22 Kda Pancornulin
Cornifin Beta
Esophagin
Database Links Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Small Proline-Rich Protein 3 antibody
Anti-22 Kda Pancornulin antibody
Anti-Cornifin Beta antibody
Anti-Esophagin antibody
Anti-SPRR3 antibody
Anti-SPRC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance