| Short Description : | Rabbit monoclonal anti-Sprouty 4 for use in WB, ICC, IF, IP and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ICC/IF/IP/FC |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | R06-8V4 |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Purification: | Affinity Chromatography |
| Dilution Range: | WB 1:500-1:1000IF 1:50-1:200IP 1:50FC 1:50-1:100 |
| Formulation: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | SPRY4 |
| Gene ID: | 81848 |
| Uniprot ID: | SPY4_HUMAN |
| Immunogen: | A synthesized peptide derived from human Sprouty 4 |
| Function | Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin. |
| Protein Name | Protein Sprouty Homolog 4Spry-4 |
| Database Links | |
| Cellular Localisation | CytoplasmCell ProjectionRuffle MembranePeripheral Membrane ProteinCytoplasmic SideFound In The Cytoplasm In Unstimulated Cells But Is Translocated To The Membrane Ruffles In Cells Stimulated With Egf (Epidermal Growth Factor)Colocalizes With Tesk1 In Vesicular Spots In The Cytoplasm |
| Alternative Antibody Names | Anti-Protein Sprouty Homolog 4 antibodyAnti-Spry-4 antibodyAnti-SPRY4 antibody |
Information sourced from Uniprot.org
