Anti-SPRED2 antibody (68-118 aa) (STJ195245)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human SPRE2 at the amino acid range 68-118
General Information
| Short Description | Rabbit polyclonal anti-Sprouty-related, EVH1 domain-containing protein 2 (68-118 aa) for use in WB and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SPRED2 |
| Gene ID | 200734 |
| Uniprot ID | SPRE2_HUMAN |
| Immunogen | Synthesized peptide derived from the human SPRE2 at the amino acid range 68-118 |
| Immunogen Region | 68-118 aa |
| Specificity | This antibody detects endogenous levels of SPRE2 at Human/Mouse/Rat |
Additional Info
| Post Translational Modifications | Phosphorylated on serine and threonine residues. Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination. Ubiquitinated.leading to degradation by the proteasome. |
| Function | Negatively regulates Ras signaling pathways and downstream activation of MAP kinases. Recruits and translocates NF1 to the cell membrane, thereby enabling NF1-dependent hydrolysis of active GTP-bound Ras to inactive GDP-bound Ras. Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2. Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells. |
| Protein Name | Sprouty-Related - Evh1 Domain-Containing Protein 2Spred-2 |
| Database Links | Reactome: R-HSA-5658442Reactome: R-HSA-5658623Reactome: R-HSA-6802953 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmic VesicleSecretory Vesicle MembraneCytoplasmDetected In The Cytoplasm Of The Stratum Spinosum CellsWhere It Is Associated With Cytoplasmic Vesicles That Are Supposed To Be Secretory Granules |
| Alternative Antibody Names | Anti-Sprouty-Related - Evh1 Domain-Containing Protein 2 antibodyAnti-Spred-2 antibodyAnti-SPRED2 antibody |
Information sourced from Uniprot.org