![Western blot analysis of lysates from wild type (WT) and SPR knockout (KO) HeLa cells, using [KO Validated] SPR Rabbit polyclonal antibody (STJ11100735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89673/358168/STJ11100735_1__80626.1713122473.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SPR knockout (KO) HeLa cells, using [KO Validated] SPR Rabbit polyclonal antibody (STJ11100735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and SPR knockout (KO) HeLa cells, using [KO Validated] SPR Rabbit polyclonal antibody (STJ11100735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89673/358168/STJ11100735_1__80626.1713122473.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SPR knockout (KO) HeLa cells, using [KO Validated] SPR Rabbit polyclonal antibody (STJ11100735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SPR |
| Gene ID: | 6697 |
| Uniprot ID: | SPRE_HUMAN |
| Immunogen Region: | 1-261 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human SPR (NP_003115.1). |
| Immunogen Sequence: | MEGGLGRAVCLLTGASRGFG RTLAPLLASLLSPGSVLVLS ARNDEALRQLEAELGAERSG LRVVRVPADLGAEAGLQQLL GALRELPRPKGLQRLLLINN AGSLGDVSKGFVDLSDSTQV NNYWALNLTSMLCLTSSVLK AFPDSPGLNRTVVNISSLCA LQPFKGWALYCAGKAARDML FQVLALEEPNVRVLNYAPGP LDTDMQQLARETSVDPDMRK GLQELKAKGKLVDCKVSAQ |
| Post Translational Modifications | In vitro phosphorylation of Ser-213 by CaMK2 does not change kinetic parameters. |
| Function | Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin. |
| Protein Name | Sepiapterin ReductaseSpr |
| Database Links | Reactome: R-HSA-1474151Reactome: R-HSA-203615 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Sepiapterin Reductase antibodyAnti-Spr antibodyAnti-SPR antibody |
Information sourced from Uniprot.org
