Anti-SPIN1 antibody (1-262) (STJ28416)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SPIN1 (1-262) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SPIN1 |
| Gene ID | 10927 |
| Uniprot ID | SPIN1_HUMAN |
| Immunogen Region | 1-262 |
| Immunogen Sequence | MKTPFGKTPGQRSRADAGHA GVSANMMKKRTSHKKHRSSV GPSKPVSQPRRNIVGCRIQH GWKEGNGPVTQWKGTVLDQV PVNPSLYLIKYDGFDCVYGL ELNKDERVSALEVLPDRVAT SRISDAHLADTMIGKAVEHM FETEDGSKDEWRGMVLARAP VMNTWFYITYEKDPVLYMYQ LLDDYKEGDLRIMPDSNDSP PAEREPGEVVDSLVGKQVEY AKEDGSKRTGMVIHQVEAK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIN1 (NP_006708.2). |
Additional Info
| Tissue Specificity | Highly expressed in ovarian cancer tissues. |
| Post Translational Modifications | Phosphorylated during oocyte meiotic maturation. |
| Function | Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and 'Lys-9' (H3K4me3K9me3) and is involved in piRNA-mediated retrotransposon silencing during spermatogenesis. Plays a key role in the initiation of the PIWIL4-piRNA pathway, a pathway that directs transposon DNA methylation and silencing in the male embryonic germ cells, by promoting recruitment of DNA methylation machinery to transposons: binds young, but not old, LINE1 transposons, which are specifically marked with H3K4me3K9me3, and promotes the recruitment of PIWIL4 and SPOCD1 to transposons, leading to piRNA-directed DNA methylation. Also recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway. Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes. May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption. |
| Protein Name | Spindlin-1Ovarian Cancer-Related ProteinSpindlin1 |
| Database Links | |
| Cellular Localisation | NucleusNucleolus |
| Alternative Antibody Names | Anti-Spindlin-1 antibodyAnti-Ovarian Cancer-Related Protein antibodyAnti-Spindlin1 antibodyAnti-SPIN1 antibodyAnti-OCR antibodyAnti-SPIN antibody |
Information sourced from Uniprot.org