Anti-SPHK2 antibody (N-Term) (STJA0005599)

SKU:
STJA0005599-100

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Host: Rabbit
Applications: WB/ICC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Sphingosine Kinase 2 (N-Term) is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Antigen Affinity Purified
Dilution Range: WB 1:250
IHC
ICC 1:100
IP 1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPHK2
Gene ID: 56848
Uniprot ID: SPHK2_HUMAN
Immunogen Region: N-Term
Specificity: The antibody detects 70 kDa* proteins corresponding to the molecular mass of SK2 on SDS-PAGE immunoblots of human recombinant SK2 and endogenous SK2 in human HeLa cells. The antibody also works for ELISA, immunoprecipitation, and immunocytochemistry.
Immunogen: SK2 (N-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human SK2. This peptide sequence is highly conserved in rat and mouse SK2 proteins, and has no homology to SK1.
Post Translational Modifications Phosphorylated by PKD on Ser-419 and Ser-421 upon PMA treatment. Phosphorylation induces export from the nucleus to the cytoplasm. Phosphorylated by MAPK1 and MAPK2 at Ser-387 and Thr-614, phosphorylation is induced by agonists such as EGF and PMA and increases kinase activity. Cleaved by CASP1 in apoptotic cells. The truncated form is released from cells.
Function Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. In contrast to prosurvival SPHK1, has a positive effect on intracellular ceramide levels, inhibits cells growth and enhances apoptosis. In mitochondria, is important for cytochrome-c oxidase assembly and mitochondrial respiration. The SPP produced in mitochondria binds PHB2 and modulates the regulation via PHB2 of complex IV assembly and respiration. In nucleus, plays a role in epigenetic regulation of gene expression. Interacts with HDAC1 and HDAC2 and, through SPP production, inhibits their enzymatic activity, preventing the removal of acetyl groups from lysine residues with histones. Up-regulates acetylation of histone H3-K9, histone H4-K5 and histone H2B-K12. In nucleus, may have an inhibitory effect on DNA synthesis and cell cycle. In mast cells, is the main regulator of SPP production which mediates calcium influx, NF-kappa-B activation, cytokine production, such as TNF and IL6, and degranulation of mast cells. In dopaminergic neurons, is involved in promoting mitochondrial functions regulating ATP and ROS levels. Also involved in the regulation of glucose and lipid metabolism.
Protein Name Sphingosine Kinase 2
Sk 2
Spk 2
Database Links Reactome: R-HSA-1660661
Cellular Localisation Cytoplasm
Nucleus
Endoplasmic Reticulum
Mitochondrion Inner Membrane
In Nucleus
Located In Nucleosomes Where It Associates With Core Histone Proteins Such As Histone 3
In Brains Of Patients With Alzheimer's Disease
May Be Preferentially Localized In The Nucleus
Cytosolic Expression Decrease Correlates With The Density Of Amyloid Deposits
In Apoptotic Cells
Colocalizes With Casp1 In Cell Membrane Where Is Cleaved And Released From Cells In An Active Form
Isoform 2: Lysosome Membrane
Alternative Antibody Names Anti-Sphingosine Kinase 2 antibody
Anti-Sk 2 antibody
Anti-Spk 2 antibody
Anti-SPHK2 antibody
Anti-SK2 antibody

Information sourced from Uniprot.org

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