Anti-SPHK2 antibody [7A5] (STJA0006219)

SKU:
STJA0006219

Current Stock:
Host: Mouse
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Sphingosine kinase 2 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 7A5
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was purified using affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPHK2
Gene ID: 56848
Uniprot ID: SPHK2_HUMAN
Specificity: This antibody detects endogenous levels of SPHK2 at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from human SPHK2
Post Translational Modifications Phosphorylated by PKD on Ser-419 and Ser-421 upon PMA treatment. Phosphorylation induces export from the nucleus to the cytoplasm. Phosphorylated by MAPK1 and MAPK2 at Ser-387 and Thr-614, phosphorylation is induced by agonists such as EGF and PMA and increases kinase activity. Cleaved by CASP1 in apoptotic cells. The truncated form is released from cells.
Function Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. In contrast to prosurvival SPHK1, has a positive effect on intracellular ceramide levels, inhibits cells growth and enhances apoptosis. In mitochondria, is important for cytochrome-c oxidase assembly and mitochondrial respiration. The SPP produced in mitochondria binds PHB2 and modulates the regulation via PHB2 of complex IV assembly and respiration. In nucleus, plays a role in epigenetic regulation of gene expression. Interacts with HDAC1 and HDAC2 and, through SPP production, inhibits their enzymatic activity, preventing the removal of acetyl groups from lysine residues with histones. Up-regulates acetylation of histone H3-K9, histone H4-K5 and histone H2B-K12. In nucleus, may have an inhibitory effect on DNA synthesis and cell cycle. In mast cells, is the main regulator of SPP production which mediates calcium influx, NF-kappa-B activation, cytokine production, such as TNF and IL6, and degranulation of mast cells. In dopaminergic neurons, is involved in promoting mitochondrial functions regulating ATP and ROS levels. Also involved in the regulation of glucose and lipid metabolism.
Protein Name Sphingosine Kinase 2
Sk 2
Spk 2
Database Links Reactome: R-HSA-1660661
Cellular Localisation Cytoplasm
Nucleus
Endoplasmic Reticulum
Mitochondrion Inner Membrane
In Nucleus
Located In Nucleosomes Where It Associates With Core Histone Proteins Such As Histone 3
In Brains Of Patients With Alzheimer's Disease
May Be Preferentially Localized In The Nucleus
Cytosolic Expression Decrease Correlates With The Density Of Amyloid Deposits
In Apoptotic Cells
Colocalizes With Casp1 In Cell Membrane Where Is Cleaved And Released From Cells In An Active Form
Isoform 2: Lysosome Membrane
Alternative Antibody Names Anti-Sphingosine Kinase 2 antibody
Anti-Sk 2 antibody
Anti-Spk 2 antibody
Anti-SPHK2 antibody
Anti-SK2 antibody

Information sourced from Uniprot.org

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